Passivating quantum dots against histag-displaying enzymes using blocking peptides: salient considerations for self-assembling quantum dot biosensors
نویسندگان
چکیده
Peptide-based passivation strategies were developed to prevent the unintended conjugation of his-tagged enzymes quantum dot (QD) biosensors, enabling application QD biosensors in complex assays such as CRISPR-based diagnostics.
منابع مشابه
Simulation of Direct Pumping of Quantum Dots in a Quantum Dot Laser
In this paper, the nonlinear rate equations governing a quantum dot laser isused to simulate the transient as well as the steady-state behaviors of the laser.Computation results show that the rate equations are capable of simulating true behaviorof a quantum dot laser. Then, the pump rates of the rate equations (which show indirectelectrical pumping of the quantum dots through a wetting layer) ...
متن کاملSelf-assembling quantum dot lattices through nucleation site engineering
The long range ordering of epitaxial semiconductor quantum dots is investigated using several crystal growth techniques on prepatterned substrates. We have developed two nucleation site control techniques: one which uses localized surface chemical potential engineering and one which uses localized surface strain engineering. We present experimental results that demonstrate the ability to create...
متن کاملSelf-assembled nanoscale biosensors based on quantum dot FRET donors.
The potential of luminescent semiconductor quantum dots (QDs) to enable development of hybrid inorganic-bioreceptor sensing materials has remained largely unrealized. We report the design, formation and testing of QD-protein assemblies that function as chemical sensors. In these assemblies, multiple copies of Escherichia coli maltose-binding protein (MBP) coordinate to each QD by a C-terminal o...
متن کاملdevelopment of different optical methods for determination of glucose using cadmium telluride quantum dots and silver nanoparticles
a simple, rapid and low-cost scanner spectroscopy method for the glucose determination by utilizing glucose oxidase and cdte/tga quantum dots as chromoionophore has been described. the detection was based on the combination of the glucose enzymatic reaction and the quenching effect of h2o2 on the cdte quantum dots (qds) photoluminescence.in this study glucose was determined by utilizing glucose...
ذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Sensors & diagnostics
سال: 2023
ISSN: ['2635-0998']
DOI: https://doi.org/10.1039/d3sd00149k